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J. Health Biol Sci. 2016; 4(3):193-197

doi:10.12662/2317-3076jhbs.v4i3.767.p193-197.2016

ORIGINAL ARTICLE

Characterization of the microbiota of the skin and oral cavity of Oreochromis niloticus Caracterização da microbiota da pele e cavidade oral de Oreochromis niloticus Edmar Maciel Lima Júnior1, Tereza de Jesus Pinheiro Gomes Bandeira2, Marcelo José Borges de Miranda3 , Guilherme Emilio Ferreira4, Ezequiel Aguiar Parente4, Nelson Sarto Piccolo5, Manoel Odorico de Moraes Filho6 1. Discente do Programa de Pós-graduação em Farmacologia da Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brasil. 2. Docente do Curso de Medicina do Centro Universitário Christus (UNICHRISTUS), Fortaleza, Ceará, Brasil. 3. Coordenador do Hospital São Marcos, Recife, Pernambuco, Brasil. 4. Discente do Curso de Medicina do Centro Universitário Christus (UNICHRISTUS), Fortaleza, Ceará, Brasil. 5. Chefe do Serviço de Cirurgia Plástica de Goiânia, Goiás, Brasil. 6. Docente do Programa de Pós-graduação em Farmacologia da Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brasil.

„•–”ƒ…– –”‘†—…–‹‘ǣ ‹•Šƒ”‡—•—ƒŽŽ›‡š’‘•‡†–‘Š‹‰Š‡”‹…”‘„‹ƒŽŽ‘ƒ†•–ŠƒŽƒ†‘”ƒ‹”ƒ‹ƒŽ•ǤŠ‡‹…”‘„‹‘–ƒ‘ˆϐ‹•Š‘•–Ž›…‘•‹•–•‘ˆPseudomonas spp., Aeromonas spp., Shewanella putrefasciens, Acinetobacter spp. and Moraxella spp. „Œ‡…–‹˜‡ǣ to analyze the oral cavity, and skin tissue microbiota on the Nile tilapia (Oreochromis niloticusȌǡƒϐ‹•Š•’‡…‹‡•”ƒ‹•‡†…‘‡”…‹ƒŽŽ›‹”ƒœ‹ŽǤMethods: Samples were collected from the oral cavity and skin of 20 Nile tilapia specimens (Oreochromis niloticus), each weighing approximately 1,000 grams. The samples were cultures for quantitative analysis on sheep blood agar (SBA) and chromID™ CPS® agar (CPS). ‡•—Ž–•ǣŽ‡˜‡†‹ˆˆ‡”‡–„ƒ…–‡”‹ƒŽ•’‡…‹‡•™‡”‡‹†‡–‹ϐ‹‡†‘ƒ†’Žƒ–‡•Ǥ ”ƒǦ‡‰ƒ–‹˜‡•’‡…‹‡•™‡”‡ the most prevalent, while gram-positive Globicatella spp, Streptococcus spp and Enterococcus faecalis were also found. Pseudomonas aeruginosa species were isolated from all samples. Gram-positive Enterococcus faecalis was found in 70 and 60% of the skin and oral samples, respectively. ‘…Ž—•‹‘: For all samples studied, the microbial load was less than 100,000 colony-forming units - CFU/g of tissue. This value is a cutoff standardized for the American ‘…‹‡–›‘ˆ‹…”‘„‹‘Ž‘‰›–‘†‹ˆˆ‡”‡–‹ƒ–‡–Š‡…ƒ—•ƒŽƒ‰‡–ˆ”‘–Š‡…‘Ž‘‹œ‡”•Ǥ Ž‹‰Š–‘ˆ–Š‹•”‡•—Ž–ƒ†…‘•‹†‡”‹‰–Š‡ƒ„•‡…‡‘ˆ‹ˆ‡…–‹‘—••‹‰•‹–Š‡ϐ‹•Š •ƒ’Ž‡•ǡ™‡…‘…Ž—†‡–Šƒ––Š‡ ˜ƒŽ—‡•ˆ‘—†‹–Š‹••–—†›”‡ϐŽ‡…–ƒ‘”ƒŽǡ‘Ǧ‹ˆ‡…–‹‘—•…‘Ž‘‹œƒ–‹‘Ȁ‹…”‘„‹‘–ƒǤ Keywords: Microbiota. Nile tilapia. Oreochromis niloticus. Fish Farming in Brazil.

Resumo Introduçãoǣ•’‡‹š‡•• ‘‘”ƒŽ‡–‡‡š’‘•–‘•ƒ…ƒ”‰ƒ•‹…”‘„‹ƒƒ•ƒ‹•‡Ž‡˜ƒ†ƒ•†‘“—‡‘•ƒ‹ƒ‹•‡–‡””ƒ‘—ƒ”Ǥ’‡”ϐ‹Ž†ƒ‹…”‘„‹‘–ƒ‡’‡‹š‡• compreende principalmente Pseudomonas spp., Aeromonas spp., Shewanella putrefasciens, Acinetobacter spp., e Moraxella spp. „Œ‡–‹˜‘ǣ analisar a microbiota da cavidade oral e do tecido da pele no Tilápia do Nilo (Oreochromis niloticus), comercialmente criado no Brasil. Métodos: Vinte espécimes de Tilápia do Nilo (Oreochromis niloticus), cada uma pesando cerca de 1.000 gramas, foram submetidas a coleta de amostras da cavidade oral e da pele. •–ƒ•ƒ‘•–”ƒ•ˆ‘”ƒ…—Ž–‹˜ƒ†ƒ•“—ƒ–‹–ƒ–‹˜ƒ‡–‡‡ž‰ƒ”•ƒ‰—‡†‡…ƒ”‡‹”‘ȋȌ‡…Š”‘ ̺̺ƒ‰ƒ”ȋȌǤ‡•—Ž–ƒ†‘•ǣ ‘”ƒ‹†‡–‹ϐ‹…ƒ†ƒ•ͳͳ diferentes espécies de bactérias em placas CPS e SBA. Os resultados mostram que bacilos gram-negativos são os mais prevalentes. Cocos gram-positivos como Globicatella spp, Streptococcus spp e Enterococcus faecalis também foram encontrados. Espécies de Pseudomonas aeruginosa foram isoladas a partir de todas as amostras. Enterococcus faecalis foi encontrado em 70 e 60% das amostras de pele e por via oral, respectivamente. Conclusão: Os resultados deste estudo mostram, para todas as amostras estudadas, uma carga de CFU de menos de 100.000 unidades formadoras de colonias - UFC / g de tecido. Este valor ±—…—–‘ˆˆ’ƒ†”‘‹œƒ†‘’‡Žƒ‘…‹‡†ƒ†‡‡”‹…ƒƒ†‡‹…”‘„‹‘Ž‘‰‹ƒǡƒϐ‹†‡†‹ˆ‡”‡…‹ƒ”‘ƒ‰‡–‡…ƒ—•ƒŽ†‘•…‘Ž‘‹œƒ†‘”‡•Ǥ‹ƒ–‡†‡•–‡•”‡•—Ž–ƒ†‘•‡ considerando a ausência de sinais infecciosos nas amostras de peixes, conclui-se que os valores CFU’s encontrados neste estudo consistem em colonização/ microbiota. ƒŽƒ˜”ƒ•Ǧ…Šƒ˜‡: Microbiota. Tilápia do Nilo. Oreochromis niloticus. Cultivo de peixe no Brasil.

INTRODUCTION O Fish farming originated 2,500 years ago in China and it was introduced in Brazil in the 1930s. The country has several ĨĂǀŽƌĂďůĞ ĐŽŶĚŝƟŽŶƐ ĨŽƌ ůĂƌŐĞͲƐĐĂůĞ ĮƐŚ ƉƌŽĚƵĐƟŽŶ͕ ƐƵĐŚ ĂƐ ůĂƌŐĞ ƚĞƌƌŝƚŽƌŝĂů ĂƌĞĂ͕ ƚƌŽƉŝĐĂů ĐůŝŵĂƚĞ͕ ĂŶĚ ĞdžƚĞŶƐŝǀĞ ƌŝǀĞƌ basins1,2. Fish farming in Brazil has been increasing in recent years. In ϮϬϭϬ͕ ƚŚĞ ĐŽƵŶƚƌLJ ǁĂƐ ƌĞƐƉŽŶƐŝďůĞ ĨŽƌ ϴϮ͘ϯй ŽĨ ĨĂƌŵĞĚ ĮƐŚ ƉƌŽĚƵĐƟŽŶ ŝŶ ^ŽƵƚŚ ŵĞƌŝĐĂ͘ ŽŵŵĞƌĐŝĂů ĨĂƌŵŝŶŐ ŽĨ EŝůĞ ƟůĂƉŝĂ ;KƌĞŽĐŚƌŽŵŝƐ ŶŝůŽƟĐƵƐͿ ŝŶĐƌĞĂƐĞĚ ϭϬϱй ďĞƚǁĞĞŶ ϮϬϬϯ ĂŶĚ ϮϬϬϵ͕ ĂŶĚ ŝƚ ŝƐ ĐƵƌƌĞŶƚůLJ ĐŽŶƐŝĚĞƌĞĚ ƚŚĞ ƐƉĞĐŝĞƐ ǁŝƚŚ ƚŚĞ ůĂƌŐĞƐƚĐŽŵŵĞƌĐŝĂůƉƌŽĚƵĐƟŽŶŝŶƌĂnjŝů3.

ĐĐŽƌĚŝŶŐ ƚŽ ƉƌĞǀŝŽƵƐůLJ ƉƵďůŝƐŚĞĚ ĚĂƚĂ ƚŚĞ ŵŝĐƌŽďŝŽƚĂ ŝŶ ĮƐŚ ĨĂƌŵŝŶŐ ŝƐ ŵĂŝŶůLJ ĐŽŵƉŽƐĞĚ ŽĨ ŐƌĂŵͲŶĞŐĂƟǀĞ ďĂĐƚĞƌŝĂ4,5,6. dŚĞLJ ĂůƐŽ ĞǀŝĚĞŶĐĞ ƚŚĂƚ ŵŝĐƌŽďŝŽƚĂ ĚŝǀĞƌƐŝƚLJ ŝŶ ĚŝīĞƌĞŶƚ EŝůĞ ƟůĂƉŝĂ ĨĂƌŵŝŶŐ ƐLJƐƚĞŵƐ͕ ƚŚĞƌĞ ŚĂǀĞ ďĞĞŶ ĨŽƵŶĚ ƐƉĞĐŝĞƐ ĨƌŽŵ ƚŚĞ ĨŽůůŽǁŝŶŐ ďĂĐƚĞƌŝĂů ĨĂŵŝůŝĞƐ͗ ŶƚĞƌŽďĂĐƚĞƌŝĂĐĞĂĞ ;ϰϱ ƐĂŵƉůĞƐͿ͕ DŝĐƌŽĐŽĐĐĂĐĞĂĞ ;ϴϳͿ͕ WƐĞƵĚŽŵŽŶĂĚĂĐĞĂĞ ;ϲϱͿ ĂŶĚ VibrioŶĂĐĞĂĞ;ϭϭϱͿϳ͕ϴ. dŚĞ ƐŝŐŶŝĮĐĂŶƚ ŝŶĐƌĞĂƐĞ ŝŶ ĂĐƟǀŝƟĞƐ ƌĞůĂƚĞĚ ƚŽ ƚŚĞ ĐƵůƟǀĂƟŽŶ ŽĨ ĮƐŚ ŽďƐĞƌǀĞĚ ŝŶ ƌĂnjŝů͕ ĞƐƉĞĐŝĂůůLJ ƚŚŽƐĞ ŚŽƐƚƐ ǁŝƚŚ ƉŽƚĞŶƟĂů ĨŽƌ ĐƵůƟǀĂƟŽŶ ĂŶĚ ŵĂƌŬĞƟŶŐ͕ ŚĂƐ ĐŽŶƐŝĚĞƌĂďůLJ enhanced the relevance of the studies carried out in order to

Corresponding Author: Tereza de Jesus Pinheiro Gomes Bandeira. Rua João Adolfo Gurgel, 133, Cocó. CEP: 60.190-060. Fortaleza,CE-Brazil. Email: [email protected] ‘ϐŽ‹…–‘ˆ‹–‡”‡•–ǣŠ‡ƒ—–Š‘”•†‡…Žƒ”‡‘…‘ϐŽ‹…–‘ˆ‹–‡”‡•–Ǥ Received: 2016 Jun 21; Revised: 2016 Jul 27, 2016 Ago 30; Accepted: 2016 Set 1

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Microbiota of the skin and oral cavity of Oreochromis niloticus

ĚĞƚĞƌŵŝŶĞ ĐŽŶƚĂŵŝŶĂƟŽŶ ŽĨ ƚŚĞƐĞ ĂŶŝŵĂůƐ ǁŝƚŚ ƉĂƚŚŽŐĞŶŝĐ microorganisms9͘ dŚƵƐ͕ ŝŶ ŝŶƚĞŶƐŝǀĞ ĮƐŚ ƉƌŽĚƵĐƟŽŶ ƐLJƐƚĞŵƐ͕ ŚĞĂůƚŚŝƐŽŶĞŽĨƚŚĞŵŽƐƚŝŵƉŽƌƚĂŶƚĂƐƉĞĐƚƐĨŽƌƚŚĞĐŽŵŵĞƌĐŝĂů ďƌĞĞĚŝŶŐŽĨĂŶLJŬŝŶĚ1.

The CFU counts were analyzed according to Isemberg and 'ĂƌĐŝĂ;ϮϬϭϬͿŝŶƌĞůĂƟŽŶƚŽƚŚĞƌĞĨĞƌĞŶĐĞǀĂůƵĞƐŽĨƋƵĂŶƟƚĂƟǀĞ ĐƵůƚƵƌĞƐ ŽĨ ǁŽƵŶĚ ƟƐƐƵĞƐ͘ dŚŝƐ ƉĂƌĂŵĞƚĞƌ ĚĞƚĞƌŵŝŶĞƐ ƚŚĂƚ ĨŽƌ ƐŝŐŶŝĮĐĂŶƚ ĐůŝŶŝĐĂů ŝƐŽůĂƚĞƐ ;ƉĂƚŚŽŐĞŶŝĐ ŽƌŐĂŶŝƐŵƐͿ͕ ƚŚĞ &h ǀĂůƵĞƐ ƐŚŽƵůĚ ďĞ ŵŽƌĞ ƚŚĂŶ ϭϬϱ &hͬŐ ŽĨ ƟƐƐƵĞ͘ ,ĞŶĐĞ͕ dŚĞ ƐƵĐĐĞƐƐ ŽĨ ĮƐŚ ĨĂƌŵŝŶŐ ĞŶƚĞƌƉƌŝƐĞƐ ĚĞƉĞŶĚƐ ŽŶ ŵĂŶLJ &h ĐŽƵŶƚƐ ďĞůŽǁ ϭϬϬ͕ϬϬϬ &hͬŐ ŽĨ ƟƐƐƵĞ ǁĞƌĞ ĐŽŶƐŝĚĞƌĞĚ ĨĂĐƚŽƌƐ͕ĂŶĚŽŶĞŽĨƚŚĞŵŽƐƚŝŵƉŽƌƚĂŶƚĐŽŶĐĞƌŶƐŝƐƚŚĞŚĞĂůƚŚ ƐƵŐŐĞƐƟǀĞŽĨďĂĐƚĞƌŝĂůĐŽůŽŶŝnjĂƟŽŶ12,13. ĐŽŶĚŝƟŽŶŽĨƚŚĞĂŶŝŵĂůƐďĞŝŶŐĐƵůƟǀĂƚĞĚ4. KƌĂů ŵƵĐƵƐ ǁĂƐ ƐĂŵƉůĞĚ ĨƌŽŵ ϮϬ ĮƐŚ ƌĂŶĚŽŵůLJ ĐŚŽƐĞŶ ĨƌŽŵ /Ŷ ĂĚĚŝƟŽŶ͕ ƚŚĞ ƐŬŝŶ ŽĨ EŝůĞ ƟůĂƉŝĂ ;KƌĞŽĐŚƌŽŵŝƐ ŶŝůŽƟĐƵƐ) is ƚŚĞƚĂŶŬƉŽƉƵůĂƟŽŶ͘DƵĐƵƐǁĂƐĐŽůůĞĐƚĞĚĨƌŽŵƚŚĞƐƵƌĨĂĐĞŽĨ ďĞŝŶŐƐƚƵĚŝĞĚďLJƌĞƐĞĂƌĐŚĞƌƐŽĨŽƵƌǁŽƌŬŝŶŐŐƌŽƵƉǁŝƚŚƚŚĞĂŝŵ the oral cavity region using sterile swabs, transferred to a tube ŽĨƵƐŝŶŐƚŚĞŵĂƐĂŶŽĐĐůƵƐŝǀĞďŝŽůŽŐŝĐĂůŐƌĂŌŝŶƚŚĞƚƌĞĂƚŵĞŶƚŽĨ ĐŽŶƚĂŝŶŝŶŐ ƐĂůŝŶĞ ĂŶĚ ŝŵŵĞĚŝĂƚĞůLJ ƐƉƌĞĂĚ ƉůĂƚĞĚ͘ dŚĞ ƐǁĂď ďƵƌŶƌĂƚƐ;ZĂƩƵƐŶŽƌǀĞŐŝĐƵƐͿtŝƐƚĂƌ͕ǁŝƚŚƚŚĞŝŶƚĞŶƟŽŶŽĨƵƐĞŝŶ ǁĂƐĮƌŵůLJƌŽůůĞĚŽǀĞƌƚŚĞĂŐĂƌƉůĂƚĞƐƵƌĨĂĐĞĂŶĚǁĂƐĐĂƌĞĨƵůůLJ ƉĂƟĞŶƚƐǁŝƚŚůĂƌŐĞĂƌĞĂƐŽĨďƵƌŶƐ;ƵŶƉƵďůŝƐŚĞĚĚĂƚĂͿ͘ ƐƚƌĞĂŬĞĚŝŶƚŚĞƉůĂƚĞĐĞŶƚĞƌĂŶĚƐƉƌĞĂĚŽŶƚŚĞĨŽƵƌƋƵĂĚƌĂŶƚƐ ƵƐŝŶŐĂƉůĂƟŶƵŵůŽŽƉ͘tŚĞŶƚŚĞƐǁĂďƐĂŵƉůĞƐǁĞƌĞĐŽůůĞĐƚĞĚ͕ dŚƵƐ͕ ŵŽŶŝƚŽƌŝŶŐ ĮƐŚ ŚĞĂůƚŚ ŝƐ ĞƐƐĞŶƟĂů ƚŽ ĞŶƐƵƌĞ ƚŚĞ ƚŚĞƌĞƐƵůƚǁĂƐƐĞŵŝͲƋƵĂŶƟƚĂƟǀĞ͕ŝ͘Ğ͕͘&hͬƐǁĂď͘ ƉƌŽĚƵĐƟŽŶ ŽĨ ŚĞĂůƚŚLJ ĂŶŝŵĂůƐ ĂŶĚ ƉƌĞǀĞŶƚ ůŽƐƐ ŝŶ ĂĐƟǀŝƚLJ10,11. ŽŶƐĞƋƵĞŶƚůLJ͕ ƚŚĞ ŽďũĞĐƟǀĞ ŽĨ ƚŚŝƐ ƐƚƵĚLJ ǁĂƐ ƚŽ ĂŶĂůLJnjĞ ƚŚĞ ŽůŽŶŝĞƐ ǁŝƚŚ ŵŽƌƉŚŽůŽŐŝĐĂůůLJ ĚŝƐƟŶĐƚ ĐŚĂƌĂĐƚĞƌŝƐƟĐƐ ŽŶ ^ ŽƌĂů ĐĂǀŝƚLJ͕ ƐŬŝŶ ĂŶĚ ƐƵďĐƵƚĂŶĞŽƵƐ ƟƐƐƵĞ ŵŝĐƌŽďŝŽƚĂ ŽĨ EŝůĞ ĂŶĚW^ĂŐĂƌǁĞƌĞƐĞůĞĐƚĞĚĂŶĚƌĞͲŝƐŽůĂƚĞĚ͘WƵƌĞĐŽůŽŶŝĞƐǁĞƌĞ ƟůĂƉŝĂ;KƌĞŽĐŚƌŽŵŝƐŶŝůŽƟĐƵƐͿĐŽŵŵĞƌĐŝĂůůLJƌĂŝƐĞĚŝŶŶĞƚƉĞŶƐ ƚŚĞŶŽďƚĂŝŶĞĚĨƌŽŵƐĞůĞĐƟǀĞĐƵůƚƵƌĞŵĞĚŝĂĂŶĚƚŚĞŶƐƵďŵŝƩĞĚ in Castanhão Reservoir, located in Jaguaribe, Ceara state, Brazil. ƚŽ ŝĚĞŶƟĮĐĂƟŽŶ ƚŚƌŽƵŐŚ 'ƌĂŵ ƐƚĂŝŶŝŶŐ ĂŶĚ ƚŚĞ ĂƵƚŽŵĂƚĞĚ s/d<ΠϮ;ďŝŽDĠƌŝĞƵdžͿŵĞƚŚŽĚ14.

MATERIAL AND METHODS This study was conducted by the Research Unit for Drug ĞǀĞůŽƉŵĞŶƚ ŽĨ &ĞĚĞƌĂů hŶŝǀĞƌƐŝƚLJ ŽĨ ĞĂƌĄ͕ Ăƚ ƚŚĞ ůŝŶŝĐĂů WĂƚŚŽůŽŐLJĞƉĂƌƚŵĞŶƚŽĨ>ĂďWĂƐƚĞƵƌ͘dŚĞƐƚƵĚLJǁĂƐĂƉƉƌŽǀĞĚ ďLJƚŚĞƚŚŝĐƐŽŵŵŝƩĞĞŽŶŶŝŵĂů^ƚƵĚŝĞƐŽĨ&ĞĚĞƌĂůhŶŝǀĞƌƐŝƚLJ ŽĨĞĂƌĄ͕ƵŶĚĞƌŶƵŵďĞƌϰϴͬϮϬϭϲ͘ dǁĞŶƚLJ ϭϬͲŵŽŶƚŚ ŽůĚ EŝůĞ ƟůĂƉŝĂ ;KƌĞŽĐŚƌŽŵŝƐ ŶŝůŽƟĐƵƐ) ǁĞŝŐŚŝŶŐ ĂƉƉƌŽdžŝŵĂƚĞůLJ ϭ͕ϬϬϬ ŐƌĂŵƐ ǁĞƌĞ ŽďƚĂŝŶĞĚ ĨƌŽŵ ŶĞƚ ƉĞŶƐ ŬĞƉƚŝŶ ĂƐƚĂŶŚĆŽ ZĞƐĞƌǀŽŝƌ͘ dŚĞƐĞĮƐŚ ǁĞƌĞƌĂŝƐĞĚ ĂƚĂ ĚĞŶƐŝƚLJŽĨϮϯĮƐŚͬϮŵ3. dŚĞƐĞƐƉĞĐŝŵĞŶƐǁĞƌĞƌĞƚƌŝĞǀĞĚĨƌŽŵŶĞƚƉĞŶƐĂŶĚŝŵŵĞĚŝĂƚĞůLJ ƉůĂĐĞĚŝŶĚŝǀŝĚƵĂůůLJŝŶƐƚĞƌŝůĞƉůĂƐƟĐďĂŐƐ͕ǁŚŝĐŚǁĞƌĞƐĞĂůĞĚĂŶĚ immersed in ice at 0 o͘hƉŽŶĂƌƌŝǀĂůĂƚƚŚĞůĂď͕ƚŚĞLJǁĞƌĞƉůĂĐĞĚ ŽŶƐƚĂŝŶůĞƐƐƐƚĞĞůƚĂďůĞƐǁŝƚŚƐŝŶŬƐ͕ǁŚŝĐŚŚĂĚďĞĞŶƉƌĞǀŝŽƵƐůLJ ĚŝƐŝŶĨĞĐƚĞĚǁŝƚŚϳϬйĞƚŚĂŶŽů͘ &ƌŽŵĞĂĐŚƟůĂƉŝĂ͕ƚǁŽϭ͘ϱdžϭ͘ϱĐŵƐŬŝŶĨƌĂŐŵĞŶƚƐǁĞƌĞŽďƚĂŝŶĞĚ͕ ǁŚŝĐŚ ǁĞŝŐŚĞĚ ĂƉƉƌŽdžŝŵĂƚĞůLJ Ϭ͘ϭϵ Ő͘ dŚĞƐĞ ĨƌĂŐŵĞŶƚƐ ǁĞƌĞ ƐĞĞĚĞĚďLJŝŵƉƌŝŶƟŶŐďŽƚŚƐŝĚĞƐŽŶƐŚĞĞƉďůŽŽĚĂŐĂƌ;^ͿĂŶĚ W^ĂŐĂƌ;ďŝŽDĠƌŝĞƵdžͿ͘ŶŽƚŚĞƌƐŬŝŶĨƌĂŐŵĞŶƚǁĂƐƚƌĂŶƐĨĞƌƌĞĚ ƚŽ ĂŶ ĞŵƉƚLJ ƐƚĞƌŝůĞ WĞƚƌŝ ĚŝƐŚ͕ ƚŽ ǁŚŝĐŚ ϭ ŵ> ŽĨ ƐƚĞƌŝůĞ ƐĂůŝŶĞ ƐŽůƵƟŽŶǁĂƐĂĚĚĞĚ͘hƐŝŶŐĂƐĐĂůƉĞů͕ƚŚŝƐĨƌĂŐŵĞŶƚǁĂƐĐƵƚŝŶƚŽ ƐĞǀĞƌĂůƉŝĞĐĞƐĂŶĚŵŝdžĞĚǁŝƚŚƚŚĞƐĂůŝŶĞƐŽůƵƟŽŶƵŶƟůŽďƚĂŝŶŝŶŐ ĂĚĞŶƐĞƐƵƐƉĞŶƐŝŽŶ͘ŶĂůŝƋƵŽƚŽĨϭϬϬђ>ŽĨƚŚŝƐƐƵƐƉĞŶƐŝŽŶǁĂƐ ƐƉƌĞĂĚ ŽŶ ^ ĂŶĚ W^ ĂŐĂƌ ǁŝƚŚ ŵŝĐƌŽďŝŽůŽŐŝĐĂů ůŽŽƉƐ͕ ĨŽƌ ĨƵƌƚŚĞƌĐŽƵŶƟŶŐŽĨĐŽůŽŶLJͲĨŽƌŵŝŶŐƵŶŝƚƐ;&hͿ͘dŚĞƌĞŵĂŝŶŝŶŐ ŵĂƚĞƌŝĂůǁĂƐƚŚĞŶƚƌĂŶƐĨĞƌƌĞĚƚŽĂƚĞƐƚƚƵďĞǁŝƚŚϯŵ>ŽĨďƌĂŝŶͲ ŚĞĂƌƚͲŝŶĨƵƐŝŽŶ;,/ͿŵĞĚŝƵŵĨŽƌĨƵƌƚŚĞƌĂŶĂůLJƐŝƐ͘dŚĞƐĞƉůĂƚĞƐ ĂŶĚƚƵďĞƐǁĞƌĞƚŚĞŶŝŶĐƵďĂƚĞĚĂƚϯϱǑ;нͬͲϭΣͿĨŽƌϮϰŚŽƵƌƐ͘

^ƚĂƟƐƟĐĂů ŶĂůLJƐŝƐ͗ dŚĞ ƌĞƐƵůƚƐ ŽďƚĂŝŶĞĚ ĨƌŽŵ ƚŚĞ ƐƚƵĚLJ ǁĞƌĞ ƐƵďũĞĐƚĞĚƚŽƚŚĞŶŽǀĂƐƚĂƟƐƟĐĂůĂŶĂůLJƐŝƐŽĨǀĂƌŝĂŶĐĞ;dĂďůĞϭͿ͘ Table 1͘DŝĐƌŽŽƌŐĂŶŝƐŵŝƐŽůĂƟŽŶƉƌŽƉŽƌƟŽŶŝŶϮϬŽƌĂůĂŶĚϮϬ ƐŬŝŶƐĂŵƉůĞƐĨƌŽŵEŝůĞƟůĂƉŝĂ;KƌĞŽĐŚƌŽŵŝƐŶŝůŽƟĐƵƐͿ͕ĐŽůůĞĐƚĞĚ ĨƌŽŵĮƐŚĂƌŵŶĞƚƉĞŶƐŝŶĂƐƚĂŶŚĆŽZĞƐĞƌǀŽŝƌ͕&ŽƌƚĂůĞnjĂ͕ĞĂƌĄ͕ ƌĂnjŝů͕ŝŶEŽǀĞŵďĞƌϮϬϭϱ͘ Microorganisms

Skin Samples(20) n (%)

Oral Cavity Samples (20) n (%)

ϮϬ;ϭϬϬͿ

ϮϬ;ϭϬϬͿ

Aeromonas sóbria

ϭϳ;ϴϱͿ

ϭϲ;ϴϬͿ

<ůĞďƐŝĞůůĂƉŶĞƵŵŽŶŝĞ

ϭϰ;ϴϱͿ

ϭϬ;ϱϬͿ

ŶƚĞƌŽĐŽĐĐƵƐĨĂĞĐĂůŝƐ

WƐĞƵĚŽŵŽŶĂƐĂĞƌƵŐŝŶŽƐĂ

ϭϰ;ϳϬͿ

ϭϮ;ϲϬͿ

ĞƌŽŵŽŶĂƐŚLJĚƌŽƉŚŝůĂ

ϴ;ϰϬͿ

ϵ;ϰϱͿ

WƌŽƚĞƵƐŵŝƌĂďŝůŝƐ

ϲ;ϯϬͿ

ϭϬ;ϱϬͿ

'ůŽďŝĐĂƚĞůůĂƐĂŶŐƵŝŶŝƐ

ϲ;ϯϬͿ

ϰ;ϮϬͿ

ĞƌŽŵŽŶĂƐǀĞƌŽŶŝŝ

ϭ;ϱͿ

ϭ;ϱͿ

^ƚƌĞƉƚŽĐŽĐĐƵƐƵďĞƌŝƐ

ϭ;ϱͿ

Ϯ;ϭϬͿ

ĂŶĚŝĚĂƉĂƌĂƉƐŝůŽƐŝƐ

ϭ;ϱͿ

ϭ;ϱͿ

^ƚƌĞƉƚŽĐŽĐĐƵƐƐƵŝƐ

ϭ;ϱͿ

ϭ;ϱͿ

ŶʹŶƵŵďĞƌŽĨƉŽƐŝƟǀĞƐĂŵƉůĞƐ ƉͲǀĂůƵĞфϬ͕ϬϬϭͲEĞƐƚĞĚŶŽǀĂĂŵŽŶŐƐƉĞĐŝĞƐŽĨďŽƚŚƐĂŵƉůĞƐ

RESULTS

dŚĞŝƐŽůĂƚĞƐŽďƚĂŝŶĞĚĨƌŽŵƚŚĞ^ĂŶĚW^ƉůĂƚĞƐĂƌĞůŝƐƚĞĚŝŶ dĂďůĞƐϭ͕ϮĂŶĚϯ͘ůůϮϬƐĂŵƉůĞƐLJŝĞůĚĞĚďĂĐƚĞƌŝĂůŐƌŽǁƚŚĂŌĞƌ ϰϴŚŽƵƌƐŽĨŝŶĐƵďĂƟŽŶ͘ůĞǀĞŶĚŝīĞƌĞŶƚďĂĐƚĞƌŝĂůƐƉĞĐŝĞƐǁĞƌĞ ŝĚĞŶƟĮĞĚŽŶW^ĂŶĚ^ƉůĂƚĞƐ͘'ƌĂŵͲŶĞŐĂƟǀĞƐƉĞĐŝĞƐǁĞƌĞ In order to calculate the total number of organisms, we used ƚŚĞ ŵŽƐƚ ƉƌĞǀĂůĞŶƚ͘ WƐĞƵĚŽŵŽŶĂƐ ĂĞƌƵŐŝŶŽƐĂ was isolated ƚŚĞĐŽůŽŶLJĐŽƵŶƚƟŵĞƐƚŚĞĚŝůƵƟŽŶĨĂĐƚŽƌ;ŚŽŵŽŐĞŶĂƚĞĚŝůƵƟŽŶ ĨƌŽŵĂůůƐĂŵƉůĞƐƐƚƵĚŝĞĚ͕ŝ͘Ğ͕͘ƉƌĞǀĂůĞŶĐĞŽĨϭϬϬй͕ĨŽůůŽǁĞĚďLJ ƉůƵƐƉůĂƚĞƐ͛ƐƵďƐĞƋƵĞŶƚĚŝůƵƟŽŶͿĚŝǀŝĚĞĚďLJƚŚĞǁĞŝŐŚƚŽĨƟƐƐƵĞ͘ Aeromonas sobria and <ůĞďƐŝĞůůĂ ƉŶĞƵŵŽŶŝĂĞ ǁŝƚŚ ϭϳй ĂŶĚ J. Health Biol Sci. 2016; 4(3): 193-197

195

Microbiota of the skin and oral cavity of Oreochromis niloticus

ϭϰйƌĞƐƉĞĐƟǀĞůLJ͘dŚĞƉŽƐŝƟǀĞĐŽĐĐŝŝƐŽůĂƚĞƐǁĞƌĞƌĞƉƌĞƐĞŶƚĞĚ͕ sobria and Klebsiella ƉŶĞƵŵŽŶŝĂĞ in decreasing order in both ŝŶ ĚĞƐĐĞŶĚŝŶŐ ŽƌĚĞƌ ŽĨ ƉƌĞǀĂůĞŶĐĞ͕ ďLJ ŶƚĞƌŽĐŽĐĐƵƐ ĨĂĞĐĂůŝƐ ŐƌŽƵƉƐŽĨƐĂŵƉůĞƐ͘ ;ϳϬйͿ͕ 'ůŽďŝĐĂƚĞůĂ ƐĂŶŐƵŝŶŝƐ ;ϯϬйͿ͕ ^ƚƌĞƉƚŽĐŽĐĐƵƐ ƵďĞƌŝƐ ;ϱйͿ YƵĂŶƟƚĂƟǀĞůLJ͕ ĂƐ ƐŚŽǁŶ ŝŶ dĂďůĞƐ Ϯ ĂŶĚ ϯ͕ W͘ ĂĞƌƵŐŝŶŽƐĂ, and ^ƚƌĞƉƚŽĐŽĐĐƵƐƐƵŝƐ;ϱйͿ͘ ďĞƐŝĚĞƐďĞŝŶŐŝƐŽůĂƚĞĚŝŶĂůůƐĂŵƉůĞƐ͕ĂůƐŽLJŝĞůĚĞĚƚŚĞŚŝŐŚĞƐƚ tŝƚŚ ƌĞƐƉĞĐƚ ƚŽ ŐƌĂŵͲƉŽƐŝƟǀĞ ďĂĐƚĞƌŝĂ͕ ŶƚĞƌŽĐŽĐĐƵƐ ĨĂĞĐĂůŝƐ ŶƵŵďĞƌŽĨ&hͬŐƌĂŵŽĨƟƐƐƵĞ͕ǁŝƚŚĂŶĂǀĞƌĂŐĞŽĨϯϭ͘ϴϱ&hͬ ŽĐĐƵƌƌĞĚ ŝŶ ϳϬ ĂŶĚ ϲϬй ŽĨ ƐŬŝŶ ĂŶĚ ŽƌĂů ĐĂǀŝƚLJ ƐĂŵƉůĞƐ͕ ŐƌĂŵŽĨƟƐƐƵĞŝŶƐŬŝŶƐĂŵƉůĞƐ͘ ƌĞƐƉĞĐƟǀĞůLJ͕ ĂŶĚ ǁĂƐ ŝŶ ƚŚŝƌĚ ƉŽƐŝƟŽŶ ŽĨ ƉƌĞǀĂůĞŶĐĞ ŝŶ ŽŵƉĂƌŝŶŐ ƚŚĞ ƐŬŝŶ ƐĂŵƉůĞƐ ǁŝƚŚ ƚŚĞ ŽƌĂů ĐĂǀŝƚLJ ƐĂŵƉůĞƐ͕ ǁĞ decreasing order. ĚŝĚŶŽƚŽďƐĞƌǀĞƐƚĂƟƐƟĐĂůůLJƐŝŐŶŝĮĐĂŶƚĚŝīĞƌĞŶĐĞƐ͕ƐŝŶĐĞƉфϬ͘Ϭϱ dĂďůĞϮƌĞƉŽƌƚƐƚŚĞ&hͬŐƌĂŵǀĂůƵĞƐŽĨƟƐƐƵĞƐĂŵƉůĞƐĂŶĚdĂďůĞ for each isolate. ϯ ƐŚŽǁƐ ƚŚĞ ƐĞŵŝͲƋƵĂŶƟƚĂƟǀĞ ƌĞƐƵůƚƐ ŽĨ ŽƌĂů ĐĂǀŝƚLJ ƐĂŵƉůĞƐ ƌĞƉŽƌƚĞĚ ĂƐ &hͬƐǁĂď͘ WƐĞƵĚŽŵŽŶĂƐ ĂĞƌƵŐŝŶŽƐĂ ƐƉĞĐŝĞƐ Yeasts of the ĂŶĚŝĚĂ ƉĂƌĂƉƐŝůŽƐŝƐ ƐƉĞĐŝĞƐ ĐŽŵƉůĞdž ǁĞƌĞ ƚŚĞ ƐŚŽǁĞĚ ƚŚĞ ŚŝŐŚĞƐƚ ŶƵŵďĞƌƐ ŽĨ &hͬŐƌĂŵ ŽĨ ƟƐƐƵĞ ;ϯϭ͘ϴϱͿ ŽŶůLJƐƉĞĐŝĞƐŽĨĨƵŶŐŝŝƐŽůĂƚĞĚŝŶďŽƚŚŐƌŽƵƉƐŽĨƐĂŵƉůĞƐ͕ǁŝƚŚ ĂŶĚŐƌĞǁŝŶϭϬϬйŽĨƚŚĞƐŬŝŶƐĂŵƉůĞƐ͕ĨŽůůŽǁĞĚďLJAeromonas ŽŶϱйƉƌĞǀĂůĞŶĐĞ;dĂďůĞϭͿ͘ Table 2. ŽůŽŶLJͲĨŽƌŵŝŶŐƵŶŝƚĐŽƵŶƚƐŽďƚĂŝŶĞĚĨƌŽŵƐŬŝŶƐĂŵƉůĞƐŽĨϮϬEŝůĞƟůĂƉŝĂ;KƌĞŽĐŚƌŽŵŝƐŶŝůŽƟĐƵƐͿĐŽůůĞĐƚĞĚĨƌŽŵŶĞƚƉĞŶƐ in Castanhão Reservoir, Fortaleza, Ceara, Brazil. Isolates

CFU/sample ϭ

















ϭϬ

ϭϭ

ϭϮ

ϭϯ

ϭϰ

ϭϱ

ϭϲ

ϭϳ

ϭϴ

ϭϵ

ϮϬ

s'1

CFU/g ƟƐƐƵĞ2

W͘ĂĞƌƵŐŝŶŽƐĂ

50

ϱϴ

30

ϭϴ

15

21

45

22

36

Ϯϴ

45

31

35

ϯϳ

Ϯϳ

ϰϴ

45

12

15

19

ϯϭ͘ϴϱ

ϭϲ͘ϳϲ

A. sóbria

0

0

15

15

12

10

6

5

16

23

ϴ

0

12

0

5

20

ϴ

ϭϴ

14

0

9.35

4.92

<͘ƉŶĞƵŵŽŶŝĂĞ

10

0

0

0

0

12

19

12

14

11

13

0

12

13

15

13

0

11

12

0

ϴ͘ϯϱ

4.39

P. mirabilis

4

6

4

4

1

0

11

0

ϴ

5

0

0

10

4

11

9

ϴ

0

6

0

4.55

2.39

͘ŚLJĚƌŽƉŚŝůĂ

10

12

0

0

0

0

0

20

6

0

0

6

0

19

0

0

0

0

ϴ

ϴ

4.45

2.34

͘ĨĂĞĐĂůŝƐ

0

0

0

0

0

0

3

6

4

5

6

5

4

3

2

2

6

6

6

4

3.1

1.63

'͘ƐĂŶŐƵŝŶŝƐ

2

2

3

3

1

2

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0.65

0.34

͘ǀĞƌŽŶŝŝ

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

ϴ

0.4

0.21

^͘ƵďĞƌŝƐ

0

0

0

0

0

0

0

0

0

0

0

0

3

2

0

0

0

0

0

0

0.25

0.13

C. parapsilosis

0

2

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0.1

0.05

^͘ƐƵŝƐ

0

0

0

0

0

0

0

0

0

0

0

0

1

0

0

0

0

0

0

0

0.05

0.03

CFU: Colony Forming Unit. 1 ǀĞƌĂŐĞ͘ 2 ZĞƐƵůƚƐĚĞƌŝǀĞĚĨƌŽŵƚŚĞĂǀĞƌĂŐĞŽĨ&hͬƐĂŵƉůĞ͘

Table 3.ŽůŽŶLJͲĨŽƌŵŝŶŐƵŶŝƚĐŽƵŶƚƐŽďƚĂŝŶĞĚĨƌŽŵŽƌĂůƐǁĂďƐŽĨϮϬEŝůĞƟůĂƉŝĂ;KƌĞŽĐŚƌŽŵŝƐŶŝůŽƟĐƵƐͿĐŽůůĞĐƚĞĚĨƌŽŵŶĞƚƉĞŶƐŝŶ Castanhão Reservoir, Fortaleza, Ceara, Brazil. CFUs /swab Isolates

AVG1 of CFU/ swab



















ϭϬ

ϭϭ

ϭϮ

ϭϯ

ϭϰ

ϭϱ

ϭϲ

ϭϳ

ϭϴ

ϭϵ

ϮϬ

W͘ĂĞƌƵŐŝŶŽƐĂ

ϴϬ

ϲϴ

61

ϳϰ

36

36

Ϯϳ

36

36

25

Ϯϳ

22

35

Ϯϳ

29

32

ϭϴ

34

Ϯϳ

29

ϯϳ͘ϵϱ

A. sóbria

0

0

ϴ

ϴ

ϭϳ

11

ϴ

5

11

10

ϴ

0

14

21

ϴ

ϭϴ

ϴ

9

12

0

ϴ͘ϴϬ

<͘ƉĞŶƵŵŽŶŝĂĞ

3

1

5

5

4

6

10

0

ϴ

6

ϭϳ

2

15

15

2

9

2

16

15

9

ϳ͘ϱϬ

P. mirailis

0

0

0

0

0

0

15

0

9

4

6

0

0

0

11

12

6

10

0

0

3.65

͘ĨĂĞĐĂůŝƐ

0

0

0

0

0

0

0

5

2

ϳ

6

2

ϴ

2

6

4

6

6

6

0

3.00

͘ŚLJĚƌŽƉŚŝůĂ

2

16

0

0

0

0

0

11

5

0

0

ϴ

0

0

0

0

0

0

0

12

Ϯ͘ϳϬ

'͘ƐĂŶŐƵŝŶŝƐ

2

3

1

2

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0.40

͘ǀĞƌŽŶŝŝ

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

ϴ

0.40

^͘ƵďĞƌŝƐ

0

0

0

0

0

0

3

0

0

0

0

0

3

0

0

0

0

0

0

0

0.30

C. parapsilosis

0

2

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0.10

^͘ƐƵŝƐ

0

0

0

0

0

0

0

0

0

0

0

0

2

0

0

0

0

0

0

0

0.10

CFU: Colony Forming Unit. ǀĞƌĂŐĞ

1

J. Health Biol Sci. 2016; 4(3):193-197

196

Microbiota of the skin and oral cavity of Oreochromis niloticus

DISCUSSION ĐĐŽƌĚŝŶŐƚŽdŝĂŵŝLJƵĞƚĂů͘;ϮϬϭϱͿ͕ƚŚĞĚĞŶƐŝƚLJŽĨďĂĐƚĞƌŝĂŝŶĮƐŚ ďŽĚŝĞƐŵĂLJĂůƐŽďĞĚƵĞƚŽĐŽŶƚĂŵŝŶĂƟŽŶĚƵƌŝŶŐŚĂŶĚůŝŶŐĂŶĚ ƐƚŽƌĂŐĞ͘dŚĞƉƌĞĚŽŵŝŶĂŶƚďĂĐƚĞƌŝĂŝƐŽůĂƚĞĚĨƌŽŵĮƐŚƐŬŝŶĂŶĚ ƐƚŽŵĂĐŚƐĂŵƉůĞƐŝŶƚŚĞŝƌƐƚƵĚLJǁĞƌĞƐƚƌĂŝŶƐŽĨ^ƚĂƉŚLJůŽĐŽĐĐƵƐ ĂƵƌĞƵƐ, Escherichia coli, WƌŽƚĞƵƐ ƐƉĞĐŝĞƐ͕ ĂĐŝůůƵƐ ƐƉĞĐŝĞƐ͕ Klebsiella ƐƉĞĐŝĞƐ͕ DŝĐƌŽĐŽĐĐƵƐ ƐƉĞĐŝĞƐ͕ ^ĞƌƌĂƟĂ ƐƉĞĐŝĞƐ͕ WƐĞƵĚŽŵŽŶĂƐ ƐƉĞĐŝĞƐ͕ Salmonella ƐƉĞĐŝĞƐ͕ ĂŶĚ ^ƚƌĞƉƚŽĐŽĐĐƵƐ ƐƉƉ15. ŽƌƌŽďŽƌĂƟŶŐƚŚĞĮŶĚŝŶŐƐŽĨdŝĂŵŝLJƵĞƚĂů͘;ϮϬϭϱͿ͕ŽƵƌƌĞƐƵůƚƐ ƐŚŽǁ ƚŚĂƚ ŐƌĂŵͲŶĞŐĂƟǀĞ ďĂĐƚĞƌŝĂ ǁĞƌĞ ŵŽƌĞ ƉƌĞǀĂůĞŶƚ ƚŚĂŶ ŐƌĂŵͲƉŽƐŝƟǀĞ ŽŶĞƐ͕ ĂŶĚ ƚŚĞ ŵŽƐƚ ĨƌĞƋƵĞŶƚ ŐƌĂŵͲŶĞŐĂƟǀĞ ƐƉĞĐŝĞƐ ǁĂƐ W͘ ĂĞƌƵŐŝŶŽƐĂ͘ ^ƉĞĐŝĞƐ ŽĨ ƚŚĞ ŐĞŶƵƐ Aeromonas were the second most frequent, with Aeromonas sobria, ĞƌŽŵŽŶĂƐ ŚLJĚƌŽƉŚŝůĂ and ĞƌŽŵŽŶĂƐ ǀĞƌŽŶŝŝ being isolated, ƌĞƉƌĞƐĞŶƟŶŐϭϳ͕ϴĂŶĚϭйŽĨƐŬŝŶƐĂŵƉůĞƐ͕ĂŶĚϭϲ͕ϵĂŶĚϭйŽĨ ŽƌĂůƐĂŵƉůĞƐ͕ƌĞƐƉĞĐƟǀĞůLJ15. dŚŝƐ ŝƐ ĂůƐŽ ƐŝŵŝůĂƌ ƚŽ ǁŚĂƚ ZŽĚƌŝŐƵĞƐ ;ϮϬϬϳͿ ƌĞƉŽƌƚĞĚ͕ ƵƐŝŶŐ ĚŝīĞƌĞŶƚ ƐĞůĞĐƟǀĞ ĐƵůƚƵƌĞ ŵĞĚŝĂ ĨŽƌ EŝůĞ ƟůĂƉŝĂ ƐĂŵƉůĞƐ ŽĨ ĨƌĞƐŚǁĂƚĞƌ ĮƐŚ ŵŝĐƌŽďŝŽƚĂ ŝŶ ƐƉĞĐŝŵĞŶƐ ĐŽůůĞĐƚĞĚ ŝŶ Ă ŐůŽďĂů ƐƚƵĚLJ͘ dŚĞ ŵŝĐƌŽďŝŽƚĂ ĐŽŶƚĂŝŶĞĚ Ă ŚŝŐŚĞƌ ĨƌĞƋƵĞŶĐLJ ŽĨ ŐƌĂŵͲ ŶĞŐĂƟǀĞ ƐƉĞĐŝĞƐ͕ ĂŶĚ ǁĂƐ ŝŶ ŵŽƐƚ ĐĂƐĞƐ ĐŽŵƉŽƐĞĚ ŽĨ ĨŽƵƌ ŐĞŶĞƌĂ͘ /Ŷ ϰϮϬ ƉůĂƚĞƐ͕ ƐŚĞ ŽďƐĞƌǀĞĚ ƚŚĞ ĨŽůůŽǁŝŶŐ ƐƉĞĐŝĞƐ͗ Aeromonas ƐƉƉ͕͘ Acinetobacter ƐƉƉ͕͘ DŝĐƌŽĐŽĐĐƵƐ ƐƉƉ͘ ;ŐƌĂŵͲ ƉŽƐŝƟǀĞͿ͕ WƐĞƵĚŽŵŽŶĂƐ ƐƉƉ͘ ĂŶĚ ƐŽŵĞ ĐŽůŝĨŽƌŵ ƐƉĞĐŝĞƐ͘ dŚĞ ĂƵƚŚŽƌ͕ ƵƐŝŶŐ ĚŝīĞƌĞŶƚ ƐĞůĞĐƟǀĞ ĐƵůƚƵƌĞ ŵĞĚŝĂ ĨŽƌ EŝůĞ ƟůĂƉŝĂ ƐĂŵƉůĞƐ͕ĂůƐŽŝĚĞŶƟĮĞĚĨŽƵƌĚŝīĞƌĞŶƚďĂĐƚĞƌŝĂůŐĞŶĞƌĂ͘/ŶϰϮϬ ƉůĂƚĞƐ͕ƐŚĞŽďƐĞƌǀĞĚƚŚĞƐĞƐƉĞĐŝĞƐĂƐĨŽůůŽǁƐ͗Aeromonas ƐƉƉ͘ Ϯϴϲ ;ϲϴ͘ϭйͿ͕ Vibrio ƐƉƉ͘ ϲϵ ;ϭϲ͘ϰйͿ͕ ŶƚĞƌŽďĂĐƚĞƌŝĂĐĞĂĞ ϱϭ ;ϭϮ͘ϮйͿĂŶĚWƐĞƵĚŽŵŽŶĂƐƐƉƉ͘ϭϰ;ϯ͘ϯйͿ͘tŚĞŶĐŽŵƉĂƌĞĚƚŽ ƚŚŝƐƐƚƵĚLJ͕ƚŚĞƌĞƐƵůƚƐĂƌĞƐŝŵŝůĂƌĞdžĐĞƉƚĨŽƌƚŚĞVibrioƐƉƉ16. ^ŝůǀĂ Ğƚ Ăů͘ ;ϮϬϭϱͿ ŝƐŽůĂƚĞĚ ĂŶĚ ŝĚĞŶƟĮĞĚ ƚŚĞ ŵĂŝŶ ďĂĐƚĞƌŝĂů ŐƌŽƵƉƐƉƌĞƐĞŶƚŝŶĨĂƌŵĞĚEŝůĞƟůĂƉŝĂ͘ŵŽŶŐƚŚĞŝƐŽůĂƚĞƐ͕ƚŚĞ most frequent genera were WƐĞƵĚŽŵŽŶĂƐ ƐƉƉ͕͘ Aeromonas ƐƉƉ͕͘ ^ƚĂƉŚLJůŽĐŽĐĐƵƐ ƐƉƉ͕͘ ĂĐŝůůƵƐ ƐƉƉ͕͘ DLJĐŽďĂĐƚĞƌŝƵŵ ƐƉƉ͕͘ DŝĐƌŽĐŽĐĐƵƐƐƉƉ͕͘ĂŶĚŽƌLJďĂĐƚĞƌŝƵŵƐƉƉϭϳ.

/ŶƌĂnjŝů͕DŽůŝŶĂƌĞƚĂů͘;ϮϬϬϯͿŵĞĂƐƵƌĞĚƚŽƚĂůďĂĐƚĞƌŝĂůŶƵŵďĞƌƐ ŝŶƚŚĞŐĂƐƚƌŽŝŶƚĞƐƟŶĂůƚƌĂĐƚŽĨƐĞŵŝͲŝŶƚĞŶƐŝǀĞůLJĐƵůƚƵƌĞĚƟůĂƉŝĂ͘ 'ƌĂŵͲŶĞŐĂƟǀĞďĂĐŝůůŝǁĞƌĞƚŚĞŵŽƐƚŝƐŽůĂƚĞĚŐƌŽƵƉŽĨďĂĐƚĞƌŝĂ and ĞƌŽŵŽŶĂƐ ŚLJĚƌŽƉŚŝůĂ, Aeromonas veronii, ƵƌŬŚŽůĚĞƌŝĂ ĐĞƉĂĐŝĂ͕ ŚƌŽŵŽďĂĐƚĞƌŝƵŵ ǀŝŽůĂĐĞƵŵ͕ ŝƚƌŽďĂĐƚĞƌ ĨƌĞƵŶĚŝŝ͕ Escherichia coli, &ůĂǀŝŵŽŶĂƐ ŽƌLJnjŝŚĂďŝƚĂŶƐ and Plesiomonas ƐŚŝŐĞůůŽŝĚĞƐ were the most frequently isolated among thanϳ. ZĞŐĂƌĚŝŶŐƚŽŐƌĂŵͲƉŽƐŝƟǀĞĐŽĐĐŝ͕ƚŚŝƐƐƚƵĚLJĚĞŵŽŶƐƚƌĂƚĞĚƚŚĞ ŝƐŽůĂƟŽŶ ŽĨ ŶƚĞƌŽĐŽĐĐƵƐ ĨĂĞĐĂůŝƐ and ^ƚƌĞƉƚŽĐŽĐĐƵƐ ƐƉĞĐŝĞƐ ĂĐĐŽƌĚŝŶŐƚŽƌĞƐƵůƚƐĨŽƵŶĚŝŶƉƌĞǀŝŽƵƐƉƵďůŝĐĂƟŽŶƐϭϴ. KƵƌ ƌĞƐƵůƚ ƉƌŽǀŝĚĞƐ ƚŚĂƚ ƚŚĞ Globicatella ŐĞŶƵƐ ǁĂƐ ƉƌĞƐĞŶƚ ŝŶ ϲ ;ϯϬйͿ ƐŬŝŶ ƐĂŵƉůĞƐ ĂŶĚ ŝŶ ϰ ;ϮϬйͿ ŽƌĂů ĐĂǀŝƚLJ ƐĂŵƉůĞƐ͘ ^ƵƌƉƌŝƐŝŶŐůLJ͕ ŝŶ Ă WƵďDĞĚ ƐƵƌǀĞLJ ǁĞ ŽŶůLJ ĨŽƵŶĚ ϭϯ ĂƌƟĐůĞƐ ŵĞŶƟŽŶŝŶŐ Globicatella sanguinis, and of these, 10 were ĂƐƐŽĐŝĂƚĞĚ ǁŝƚŚ ŚƵŵĂŶ ĚŝƐĞĂƐĞƐ ĂŶĚ ŽŶůLJ ϯ ŝŶ ĂŶŝŵĂůƐ ;Ϯ ŝŶ ƐŚĞĞƉĂŶĚϭŝŶŚŽƌƐĞƐͿ19,20,21. ůƚŚŽƵŐŚ ƐŽŵĞ ĂƵƚŚŽƌƐ ŚĂǀĞ ƌĞƉŽƌƚĞĚ ƐŝŐŶŝĮĐĂŶƚ ŝƐŽůĂƟŽŶ ŽĨ ĂŶĚŝĚĂ ƐƉĞĐŝĞƐ ĐŽůůĞĐƚĞĚ ĨƌŽŵ ĂƋƵĂƟĐ ĂŶŝŵĂůƐ͕ ŽƵƌ ƐƚƵĚLJ ƐŚŽǁĞĚ ŽŶůLJ ƚǁŽ ƐƉĞĐŝĞƐ ŽĨ ƚŚĞ ĂŶĚŝĚĂ ƉĂƌĂƉƐŝůŽƐŝƐ ƐƉĞĐŝĞƐ ĐŽŵƉůĞdž͕ŽŶĞƌĞĐŽǀĞƌĞĚĨƌŽŵƐŬŝŶƐĂŵƉůĞƐĂŶĚƚŚĞŽƚŚĞƌĨƌŽŵ the oral cavity swab22,23,24.

CONCLUSION ůƚŚŽƵŐŚ ƐŽŵĞ ƐĐŝĞŶƟĮĐ ƉƵďůŝĐĂƟŽŶƐ ŚĂǀĞ ƐŚŽǁŶ ƚŚĂƚ ƚŚĞ ƌŽůĞ ŽĨ ŵĂŶLJ ŽĨ ƚŚĞƐĞ ĮƐŚͲĂƐƐŽĐŝĂƚĞĚ ďĂĐƚĞƌŝĂ ŝƐ ƵŶĐůĞĂƌ ĂŶĚ ƚŚĞ ƌĞůĂƟŽŶƐŚŝƉ ďĂĐƚĞƌŝĂͬĮƐŚ ƵŶĐĞƌƚĂŝŶ͕ ǁĞ ƉĞƌĨŽƌŵĞĚ ŝŶ ŽƵƌ ǁŽƌŬĂƋƵĂŶƟƚĂƟǀĞĐƵůƚƵƌĞƚŚĂƚƐŚŽǁƐŝŶĂůůƐĂŵƉůĞƐƐƚƵĚŝĞĚ͕ Ă &h ůŽĂĚ ůĞƐƐ ƚŚĂŶ ϭϬϬ͕ϬϬϬ &hͬŐ ŽĨ ƟƐƐƵĞ͘ dŚŝƐ ǀĂůƵĞ ŝƐ Ă ĐƵƚŽīƐƚĂŶĚĂƌĚŝnjĞĚďLJƚŚĞŵĞƌŝĐĂŶ^ŽĐŝĞƚLJŽĨDŝĐƌŽďŝŽůŽŐLJƚŽ ĚŝīĞƌĞŶƟĂƚĞƚŚĞĐĂƵƐĂůĂŐĞŶƚĨƌŽŵĐŽůŽŶŝnjĞƌŝƐŽůĂƚĞƐŝŶŚƵŵĂŶ wounds. In light of these results and considering the absence of ŝŶĨĞĐƟŽƵƐƐŝŐŶƐŝŶƚŚĞĮƐŚƐĂŵƉůĞƐ͕ǁĞĐŽŶĐůƵĚĞƚŚĂƚƚŚĞ&h ǀĂůƵĞƐĨŽƵŶĚŝŶƚŚŝƐƐƚƵĚLJŝŶĚŝĐĂƚĞƚŚĞƉƌĞƐĞŶĐĞŽĨŶŽƌŵĂů͕ŶŽŶͲ ŝŶĨĞĐƟŽƵƐŵŝĐƌŽďŝŽƚĂ͘

REFERENCES ϭ͘ ĂŐŽ ͘͘ ŶĄůŝƐĞ ƉĂƌĂƐŝƚŽůŽŐŝĐĂů Ğ ŵŝĐƌŽďŝŽůſŐŝĐĂ ĚĞ ƟůĄƉŝĂƐ ĚŽ EŝůŽ ;KƌĞŽĐŚƌŽŵŝƐ ŶŝůŽƟĐƵƐͿ͕ ĐƌŝĂĚĂƐ Ğŵ ƚĂŶƋƵĞƐͲƌĞĚĞ ŶŽ ƌĞƐĞƌǀĂƚſƌŝŽ ĚĞ ŐƵĂ sĞƌŵĞůŚĂͲ ^W Ğ ƐƵĂƐ ŝŶƚĞƌͲƌĞůĂĕƁĞƐ ĐŽŵ ĂƐ ǀĂƌŝĄǀĞŝƐ ŝŵƵŶŽůſŐŝĐĂƐ Ğ ĨĂƐĞ ĚĞ ĐƌŝĂĕĆŽ΀ĚŝƐƐĞƌƚĂƟŽŶ΁͘ŽƚƵĐĂƚƵ;^WͿ͗hE^W͖ϮϬϭϮ͘ Ϯ͘ ŚĞĐŚŝŵ͕ &͘͘ ĂƌĂĐƚĞƌşƐƟĐĂƐ ŵŽƌĨŽůſŐŝĐĂƐ ĚŽ ĞƉŝƚĠůŝŽ ŝŶƚĞƐƟŶĂů Ğ ĚĞƐĞŵƉĞŶŚŽ ĚĞ ƟůĄƉŝĂ ĚŽ EŝůŽ͕ KƌĞŽĐŚƌŽŵŝƐ ŶŝůŽƟĐƵƐ͕ ƐƵƉůĞŵĞŶƚĂĚĂ ĐŽŵ ŵĂŶĂŶŽůŝŐŽƐƐĂĐĂƌşĚĞŽ;DK^Ϳ΀ĚŝƐƐĞƌƚĂƟŽŶ΁͘ŽŝƐsŝnjŝŶŚŽƐ΀WZ΁͗hd&WZ͖ϮϬϭϯ͘ ϯ͘^ŽƵƐĂ͘͘>͘DĂŶĂŶŽůŝŐŽƐƐĂĐĂƌşĚĞŽĞͲŐůƵĐĂŶŽŶĂƐƵƉůĞŵĞŶƚĂĕĆŽĚŝĞƚĄƌŝĂ ƉĂƌĂũƵǀĞŶŝƐĚĞƟůĄƉŝĂĚŽEŝůŽŵĂŶƟĚŽƐĞŵƚĂŶƋƵĞͲƌĞĚĞ΀ƚŚĞƐŝƐ΁͘:ĂďŽƟĐĂďĂů ΀^W΁͗hE^W͖ϮϬϭϬ͘ ϰ͘ WĂǀĂŶĞůůŝ͕ '͘ ͖͘ ŝƌĂƐ͕ :͘ ͖͘ dĂŬĞŵŽƚŽ͕ Z͘ D͘ ;ϮϬϬϴͿ͘ ŽĞŶĕĂƐ ĚĞ ƉĞŝdžĞƐ͗ ƉƌŽĮůĂdžŝĂ͕ĚŝĂŐŶſƐƟĐŽĞƚƌĂƚĂŵĞŶƚŽ͘ϯƌĚ͘DĂƌŝŶŐĄ͗ĚƵĞŵ͖ϮϬϬϴ͘ ϱ͘ >ĂŶnjĂƌŝŶ D͕͘ ůŵĞŝĚĂ &ŝůŚŽ ͘^͕͘ ZŝƩĞƌ ͘K͕͘ DĞůůŽ ͕͘͘ ŽƌƌġĂ ͘^͘^͕͘ /ŐŶĄĐŝŽ͘D͘^͘KĐŽƌƌġŶĐŝĂĚĞAeromonas ƐƉ͘ĞŵŝĐƌŽƌŐĂŶŝƐŵŽƐƉƐŝĐƌŽƚƌſĮĐŽƐĞ

ĞƐƟŵĂƟǀĂĚŽƉƌĂnjŽĚĞǀĂůŝĚĂĚĞĐŽŵĞƌĐŝĂůĚĞĮůĠĚĞƉŝŶƚĂĚŽ;WƐĞƵĚŽƉůĂƚLJƐƚŽŵĂ ĐŽƌƵƐĐĂŶƐͿŵĂŶƟĚŽƐƐŽďƌĞĨƌŝŐĞƌĂĕĆŽ͘ƌƋ͘ƌĂƐ͘DĞĚ͘sĞƚ͘ŽŽƚĞĐ͘ϮϬϭϭĞĐ͖ ϲϯ;ϲͿ͗ϭϱϰϭͲϭϱϰϲ͘ĚŽŝ͗ŚƩƉ͗ͬͬĚdž͘ĚŽŝ͘ŽƌŐͬϭϬ͘ϭϱϵϬͬ^ϬϭϬϮͲϬϵϯϱϮϬϭϭϬϬϬϲϬϬϬϯϱ͘ ϲ͘'ŝĂƚƐŝƐ͕͘^ŝƉŬĞŵĂ͕͘^ŵŝĚƚ,͕͘,ĞŝůŝŐ,͕͘ĞŶǀĞŶƵƟ'͕͘sĞƌƌĞƚŚ:͕͘sĞƌĚĞŐĞŵ D͘ /ŵƉĂĐƚ ŽĨ ƌĞĂƌŝŶŐ ĞŶǀŝƌŽŶŵĞŶƚ ŽŶ ƚŚĞ ĚĞǀĞůŽƉŵĞŶƚ ŽĨ ŐƵƚ ŵŝĐƌŽďŝŽƚĂ ŝŶ ƟůĂƉŝĂůĂƌǀĂĞ͘^ĐŝĞŶƟĨĐZĞƉƉŽƌƚƐ͘ϮϬϭϱĞĐϭϭ΀ĂĐĐĞƐƐϮϬϭϲƉƌϮϬ΁͖ϱ;ϭϴϮϬϲͿ͗ ϭͲϭϱ͘ ǀĂŝůĂďůĞ ĨƌŽŵ͗ ŚƩƉ͗ͬͬǁǁǁ͘ŶĂƚƵƌĞ͘ĐŽŵͬĂƌƟĐůĞƐͬƐƌĞƉϭϴϮϬϲ͘ K/͗ ϭϬ͘ϭϬϯϴͬƐƌĞƉϭϴϮϬϲ͘ ϳ͘DŽůŝŶĂƌŝ>͘D͕͘^ĐŽĂƌŝƐ͘K͕͘WĞĚƌŽƐŽZ͕͘͘ŝƩĞŶĐŽƵƌƚE͘>͘Z͕͘EĂŬĂŵƵƌĂ͘s͕͘ hĞĚĂŶĂŬĂŵƵƌĂ d͕͘ Ğƚ Ăů͘ ĂĐƚĞƌŝĂů ŵŝĐƌŽďŝŽƚĂ ŝŶ ƚŚĞ ŐĂƐƚƌŽŝŶƚĞƐƟŶĂů ƚƌĂĐƚ ŽĨ EŝůĞ ƟůĂƉŝĂ͕ KƌĞŽĐŚƌŽŵŝƐ ŶŝůŽƟĐƵƐ ĐƵůƚƵƌĞĚ ŝŶ Ă ƐĞŵŝͲŝŶƚĞŶƐŝǀĞ ƐLJƐƚĞŵ͘ ĐƚĂ ^ĐŝĞŶƟĂƌƵŵ͘ŝŽůŽŐŝĐĂů^ĐŝĞŶĐĞƐ͘ϮϬϬϯ͖Ϯϱ;ϮͿ͗ϮϲϳͲϮϳϭ͘ ϴ͘WĂƌŬŝŶŐŬŝŶŐZ͕͘WĂůŵĂW͕͘hƐĞƌŽZ͘YƵĂŶƟƚĂƟǀĞĂŶĚƋƵĂůŝƚĂƟǀĞĂŶĂůLJƐĞƐŽĨƚŚĞ ďĂĐƚĞƌŝĂůŵŝĐƌŽďŝŽƚĂŽĨƟůĂƉŝĂ;KƌĞŽĐŚƌŽŵŝƐŶŝůŽƟĐƵƐͿĐƵůƚƵƌĞĚŝŶĞĂƌƚŚĞŶƉŽŶĚƐ ŝŶ ƚŚĞ WŚŝůŝƉƉŝŶĞƐ͘ tŽƌůĚ DŝĐƌŽďŝŽů ŝŽƚĞĐŚŶŽů͘ ϮϬϭϱ &Ğǀ͖ ϯϭ;ϮͿ͗ϮϲϱͲϮϳϱ͘ ĚŽŝ͗

J. Health Biol Sci. 2016; 4(3):193-197

ϭϵϳ

Microbiota of the skin and oral cavity of Oreochromis niloticus

ϭϬ͘ϭϬϬϳͬƐϭϭϮϳϰͲϬϭϰͲϭϳϱϴͲϭ͘WƵďDĞĚWD/͗Ϯϱϱϱϱϯϳϱ͘ ϵ͘ĂƌŽŶLJ'͘D͕͘dĂǀĂƌĞƐ'͕͘͘ƐƐŝƐ'͘͘E͕͘>ƵnjZ͘<͕͘&ŝŐƵĞŝƌĞĚŽ,͕͘͘>ĞĂů͘͘ EĞǁ ŚŽƐƚƐ ĂŶĚ ŐĞŶĞƟĐ ĚŝǀĞƌƐŝƚLJ ŽĨ &ůĂǀŽďĂƚĞƌŝƵŵ ĐŽůƵŵŶĂƌĞ ŝƐŽůĂƚĞĚ ĨƌŽŵ ƌĂnjŝůŝĂŶEĂƟǀĞƐƉĞĐŝĞƐĂŶĚEŝůĞƟůĂƉŝĂ͘ŝƐƋƵĂƚKƌŐĂŶ͘ϮϬϭϱEŽǀϭϳ͖ϭϭϳ;ϭͿ͗ϭͲ ϭϭ͘ĚŽŝ͗ϭϬ͘ϯϯϱϰͬĚĂŽϬϮϵϯϭ͘ ϭϬ͘ƵƐƟŶ͕͘dŚĞďĂĐƚĞƌŝĂůŵŝĐƌŽŇŽƌĂŽĨĮƐŚ͕ƌĞǀŝƐĞĚ͘^ĐŝĞŶƟĮĐtŽƌůĚ:ŽƵƌŶĂů͘ ϮϬϬϲƵŐϭϭ͘ϲ͗ϵϯϭʹϵϰϱ͘ ϭϭ͘ ,ƵŝĐĂďͲWĞĐŚ ͘'͕͘ >ĂŶĚĞƌŽƐͲ^ĄŶĐŚĞnj ͕͘ ĂƐƚĂŹĞĚĂͲŚĄǀĞnj D͘Z͕͘ >ĂŶŐŽͲZĞLJŶŽƐŽ &͕͘ >ſƉĞnjͲŽůůĂĚŽ ͘:͕͘ WůĂƚĂƐ ZŽƐĂĚŽ ͘͘ ƵƌƌĞŶƚ ƐƚĂƚĞ ŽĨ ďĂĐƚĞƌŝĂ ƉĂƚŚŽŐĞŶŝĐŝƚLJ ĂŶĚ ƚŚĞŝƌ ƌĞůĂƟŽƐŚŝƉ ǁŝƚŚ ŚŽƐƚ ĂŶĚ ĞŶǀŝƌŽŶŵĞŶƚ ŝŶ ƟůĂƉŝĂ KƌĞŽĐŚƌŽŵŝƐ ŶŝůŽƟĐƵƐ͘ ƋƵĂĐ͘ ϮϬϭϲ͖ ϳ;ϱͿ͗ϭͲϭϬ͘ ĚŽŝ͗ ϭϬ͘ϰϭϳϮͬϮϭϱϱͲ ϵϱϰϲ͘ϭϬϬϬϰϮϴ͘ ϭϮ͘ŐġŶĐŝĂEĂĐŝŽŶĂůĚĞsŝŐŝůąŶĐŝĂ^ĂŶŝƚĄƌŝĂ͘DŝĐƌŽďŝŽůŽŐŝĂĐůşŶŝĐĂƉĂƌĂŽĐŽŶƚƌŽůĞ ĚĞ ŝŶĨĞĐĕĆŽ ƌĞůĂĐŝŽŶĂĚĂ ă ĂƐƐŝƐƚġŶĐŝĂ ă ƐĂƷĚĞ͘ DſĚƵůŽ ϰ͗ ƉƌŽĐĞĚŝŵĞŶƚŽƐ ůĂďŽƌĂƚŽƌŝĂŝƐ͗ ĚĂ ƌĞƋƵŝƐŝĕĆŽ ĚŽ ĞdžĂŵĞ ă ĂŶĄůŝƐĞ ŵŝĐƌŽďŝŽůſŐŝĐĂ Ğ ůĂƵĚŽ ĮŶĂů͘ ƌĂƐşůŝĂ͗Es/^͖ϮϬϭϯ͘ϵϱƉ͘ ϭϯ͘'ĂƌĐŝĂ͕>͘^͖͘/ƐĞŶďĞƌŐ,͘͘ůŝŶŝĐĂůDŝĐƌŽďŝŽůŽŐLJWƌŽĐĞĚƵƌĞƐ,ĂŶĚŬ͘ϯƌĚ͘ ĞĚ͘tĂƐŚŝŶŐƚŽŶ͗ŵĞƌŝĐĂŶ^ŽĐŝĞƚLJĨŽƌDŝĐƌŽďŝŽůŽŐLJ͖ϮϬϭϬ͘ ϭϰ͘WŝŶĐƵƐ͕͘,͘DŝĐƌŽďŝĂůŝĚĞŶƟĮĐĂƟŽŶƵƐŝŶŐƚŚĞďŝŽDĞƌŝĞƵdžs/d<Ϯ^LJƐƚĞŵ͘ /Ŷ͗DŝůůĞƌ͕D͘ŶĐLJĐůŽƉĞĚŝĂŽĨZĂƉŝĚDŝĐƌŽďŝŽůŽŐŝĐĂůDĞƚŚŽĚƐ͘ĞƚŚĞƐĚĂ͗Wͬ ,/͖ϮϬϬϲ͘ƉϭͲϯϮ͘ ϭϱ͘ dŝĂŵŝLJƵ͕ ͘D͕͘ ^ŽůĂĚŽLJĞ͕ D͘K͕͘ ĚĞŐďŽLJĞŐĂ͕ d͘d͘ ĂŶĚ ĚĞƚŽŶĂ͕ D͘K͘ KĐĐƵƌƌĞŶĐĞ ĂŶĚ ŶƟďŝŽƟĐ ^ĞŶƐŝƟǀŝƚLJ ŽĨ ĂĐƚĞƌŝĂů ^ƚƌĂŝŶƐ /ƐŽůĂƚĞĚ ĨƌŽŵ EŝůĞ dŝůĂƉŝĂ͕KƌĞŽĐŚƌŽŵŝƐŶŝůŽƟĐƵƐKďƚĂŝŶĞĚŝŶ/ďĂĚĂŶ͕^ŽƵƚŚǁĞƐƚEŝŐĞƌŝĂ͘:ŽƵƌŶĂů ŽĨŝŽƐĐŝĞŶĐĞƐĂŶĚDĞĚŝĐŝŶĞƐ͘ϮϬϭϱ͖ϯ͗ϭϵͲϮϲ͘ĚŽŝ͗ŚƩƉ͗ͬͬĚdž͘ĚŽŝ͘ŽƌŐͬϭϬ͘ϰϮϯϲͬ jbm.2015.35003. ϭϲ͘ ZŽĚƌŝŐƵĞƐ͕ ͘ WĞƐƋƵŝƐĂ ĚĞ Aeromonas ƐƉƉ͘ Ğŵ ƟůĄƉŝĂ ;Oreochromis ŶŝůŽƟĐƵƐͿ͕ĐƵůƟǀĂĚĂŶŽĞƐƚĂĚŽĚŽZŝŽĚĞ:ĂŶĞŝƌŽͲƌĂƐŝů͗ŝƐŽůĂŵĞŶƚŽ͕ŝĚĞŶƟĮĐĂĕĆŽ ĚĞ ĞƐƉĠĐŝĞƐ Ğ ĂǀĂůŝĂĕĆŽ ĚĂ ƐĞŶƐŝďŝůŝĚĂĚĞ ĂŶƟŵŝĐƌŽďŝĂŶĂ ΀ƚĞƐĞ΁͘ EŝƚĞƌſŝ ;Z:Ϳ͗ hŶŝǀĞƌƐŝĚĂĚĞ&ĞĚĞƌĂů&ůƵŵŝŶĞŶƐĞ͖ϮϬϬϳ͘ ϭϳ͘ ^ŝůǀĂ͕ :͘>͘^͖͘ ĂǀĂůĐĂŶƚĞ͕ ͘,͖͘ ĂƌǀĂůŚŽ͕ &͘͘d͖͘ sŝĞŝƌĂ͕ Z͘,͘^͘&͖͘ ^Ą D͘s͘͘

ĂŶĚ ^ŽƵƐĂ͕ K͘s͘ ƋƵĂƟĐ ŵŝĐƌŽďŝŽƚĂ ĚŝǀĞƌƐŝƚLJ ŝŶ ƚŚĞ ĐƵůƚƵƌĞ ŽĨ EŝůĞ ƟůĂƉŝĂ ;KƌĞŽĐŚƌŽŵŝƐ ŶŝůŽƟĐƵƐͿ ƵƐŝŶŐ ďŝŽŇŽĐƐ Žƌ ƉĞƌŝƉŚLJƚŽŶ͗ ǀŝƌƵůĞŶĐĞ ĨĂĐƚŽƌƐ ĂŶĚ ďŝŽĮůŵĨŽƌŵĂƟŽŶ͘ĐƚĂ^ĐŝĞŶƟĂƌƵŵ͘ŶŝŵĂů^ĐŝĞŶĐĞƐ͘ϮϬϭϲ:ƵůͲ^ĞƉ͖ϯϴ;ϯͿ͗ϮϯϯͲ Ϯϰϭ͘ĚŽŝ͗ŚƩƉ͗ͬͬĚdž͘ĚŽŝ͘ŽƌŐͬϭϬ͘ϰϬϮϱͬĂĐƚĂƐĐŝĂŶŝŵƐĐŝ͘ǀϯϴŝϯ͘ϯϭϵϭϬ͘ ϭϴ͘ŶƐŚĂƌLJ,͕͘<ƵƌŶŝĂǁĂŶZ͕͘͘^ƌŝǁƵůĂŶ^͕͘ZĂŵůŝZ͕͘ĂdžĂ͘s͘/ƐŽůĂƟŽŶĂŶĚ ŵŽůĞĐƵůĂƌŝĚĞŶƟĮĐĂƟŽŶŽĨƚŚĞĞƟŽůŽŐŝĐĂůĂŐĞŶƚƐŽĨ^ƚƌĞƉƚŽĐŽĐĐƵƐŝŶEŝůĞƟůĂƉŝĂ ;KƌĞŽĐŚƌŽŵŝƐŶŝůŽƟĐƵƐͿĐƵůƚƵƌĞĚŝŶŶĞƚĐĂŐĞƐŝŶ>ĂŬĞ^ĞŶƚĂŶŝ͕WĂƉƵĂ͕/ŶĚŽŶĞƐŝĂ͘ ^ƉƌŝŶŐĞƌƉůƵƐ͘ ϮϬϭϰ KĐƚ Ϯϰ͖ ϯ;ϲϮϳͿ͗ϭͲϭϭ͘ ĚŽŝ͗ ϭϬ͘ϭϭϴϲͬϮϭϵϯͲϭϴϬϭͲϯͲϲϮϳ͘ WƵďDĞĚWD/͗ϮϱϯϵϮϳϵϳ͘ ϭϵ͘ůƐŝŶŐŚŽƌƐƚd͘͘&ŝƌƐƚĐĂƐĞƐŽĨĂŶŝŵĂůĚŝƐĞĂƐĞƐƉƵďůŝƐŚĞĚƐŝŶĐĞϮϬϬϬ͘sĞƚY͘ ϮϬϬϯ͖Ϯϱ;ϰͿ͗ϭϲϱͲϭϲϵ͘ ĚŽŝ͗ ϭϬ͘ϭϬϴϬͬϬϭϲϱϮϭϳϲ͘ϮϬϬϯ͘ϵϲϵϱϭϲϬ͘ WƵďDĞĚ WD/͗ ϭϰϳϭϰϳϰϬ͘ ϮϬ͘sĞůĂ͘/͕͘&ĞƌŶĄŶĚĞnj͕͘>ĂƐ,ĞƌĂƐ͕͘>ĂǁƐŽŶW͕͘͘ŽŵşŶŐƵĞnj>͕͘ŽůůŝŶƐD͕͘͘ &ĞƌŶĂŶĚĞnjͲ'ĂƌĂLJnjĂďĂů :͘&͘ DĞŶŝŶŐŽĞŶĐĞƉŚĂůŝƟƐ ĂƐƐŽĐŝĂƚĞĚ ǁŝƚŚ Globicatella ƐĂŶŐƵŝŶŝƐ ŝŶĨĞĐƟŽŶ ŝŶ ůĂŵďƐ͘ : ůŝŶ DŝĐƌŽďŝŽů͘ ϮϬϬϬ EŽǀ͖ ϯϴ;ϭϭͿ͗ϰϮϱϰͲϰϮϱϱ͘ WƵďDĞĚĞŶƚƌĂůWD/͗WDϴϳϱϳϱ͘ Ϯϭ͘ŽůůŝŶƐD͕͘͘ZŽĚƌŝŐƵĞnj:͘D͕͘>ĂǁƐŽŶW͕͘͘&ĂůƐĞŶ͕͘&ŽƐƚĞƌ'͘ŚĂƌĂĐƚĞƌŝnjĂƟŽŶ ŽĨĂŶŽǀĞůŐƌĂŵͲƉŽƐŝƟǀĞ͕ĐĂƚĂůĂƐĞͲŶĞŐĂƟǀĞĐŽĐĐƵƐĨƌŽŵŚŽƌƐĞƐ͗ĚĞƐĐƌŝƉƟŽŶŽĨ ƌĞŵŽĐŽĐĐƵƐĐŽůĞŽĐŽůĂŐĞŶ͘ŶŽǀ͕͘ƐƉ͘ŶŽǀ͘/Ŷƚ:^LJƐƚĂĐƚĞƌŝŽů͘ϭϵϵϵKĐƚ͖ϰϵ;Wƚ ϰͿ͗ϭϯϴϭͲϭϯϴϱ͘ĚŽŝ͗ϭϬ͘ϭϬϵϵͬϬϬϮϬϳϳϭϯͲϰϵͲϰͲϭϯϴϭ͘WƵďDĞĚWD/͗ϭϬϱϱϱϯϭϲ͘ ϮϮ͘ ƌŝůŚĂŶƚĞ Z͘^͕͘ ĂƐƚĞůŽͲƌĂŶĐŽ ͘^͕͘ ^ŽĂƌĞƐ͕ '͕͘͘ ƐƚĞƚĞͲDĞĚƌĂŶŽ ͘:͕͘ DŽŶƚĞŝƌŽ ͘:͕͘ ŽƌĚĞŝƌŽ Z͕͘͘ Ğƚ Ăů͘ ŚĂƌĂĐƚĞƌŝnjĂƟŽŶ ŽĨ ƚŚĞ ŐĂƐƚƌŽŝŶƚĞƐƟŶĂů LJĞĂƐƚ ŵŝĐƌŽďŝŽƚĂ ŽĨ ĐŽĐŬĂƟĞůƐ ;ELJŵƉŚŝĐƵƐ ŚŽůůĂŶĚŝĐƵƐͿ͗ Ă ƉŽƚĞŶƟĂů ŚĂnjĂƌĚ ƚŽ ŚƵŵĂŶ ŚĞĂůƚŚ͘ : DĞĚ DŝĐƌŽďŝŽů͘ ϮϬϭϬ :ƵŶ͖ ϱϵ;Wƚ ϲͿ͗ ϳϭϴʹϳϮϯ͘ ĚŽŝ͗ ϭϬ͘ϭϬϵϵͬ ũŵŵ͘Ϭ͘ϬϭϳϰϮϲͲϬ͘WƵďDĞĚWD/͗ϮϬϭϱϬϯϭϴ͘ Ϯϯ͘ƌŝůŚĂŶƚĞZ͘^͕͘:ĞƐƵƐ^͘Z͘d͕͘^ŽƵnjĂ͘D͕͘͘͘dĞŝdžĞŝƌĂ͕͘͘ƌŝƚŽD͘Z͕͘ĂŶĚĞŝƌĂ ^͘W͕͘ Ğƚ Ăů͘ ĂŶĚŝĚĂ ƉĂƌĂƉƐŝůŽƐŝƐ ĐŽŵƉůĞdž ĨƌŽŵ ĂŶŝŵĂůƐ ĂŶĚ ŝƚƐ ĂŶƟĨƵŶŐĂů ƐƵƐĐĞƉƟďŝůŝƚLJ ĂŶĚ ǀŝƌƵůĞŶĐĞ ĂƩƌŝďƵƚĞƐ͘  DĞĚ DŝĐƌŽďŝŽů͘ ϮϬϭϰ EŽǀ͖ ϲϯ;Wƚ ϭϭͿ͗ ϭϱϲϴͲϭϱϳϮ͘ĚŽŝ͗ϭϬ͘ϭϬϵϵͬũŵŵ͘Ϭ͘ϬϳϲϮϭϲͲϬ͘WƵďDĞĚWD/͗ϮϱϭϵϬϳϯϲ͘ Ϯϰ͘^ŝĚƌŝŵ:͘:͕͘ĂƌǀĂůŚŽs͘>͕͘ĂƐƚĞůŽͲƌĂŶĐŽ͘^͕͘ƌŝůŚĂŶƚĞZ͘^͕͘ĂŶĚĞŝƌĂd͘:͕͘ ŽƌĚĞŝƌŽZ͕͘͘ĞƚĂů͘zĞĂƐƚŵŝĐƌŽďŝŽƚĂŽĨŶĂƚƵƌĂůĐĂǀŝƟĞƐŽĨŵĂŶĂƚĞĞƐ;dƌŝĐŚĞĐŚƵƐ inunguis and Trichechus manatus ) in Brazil and its relevance for animal health ĂŶĚŵĂŶĂŐĞŵĞŶƚŝŶĐĂƉƟǀŝƚLJ͘ĂŶ:DŝĐƌŽďŝŽů͘ϮϬϭϱKĐƚ͘ϲϭ;ϭϬͿ͗ϳϲϯͲϳϲϵ͘

‘‘…‹–ƒ”‡•–‡ƒ”–‹‰‘Ȁ ‘™–‘…‹–‡–Š‹•ƒ”–‹…Ž‡ǣ Lima EM Júnior, Bandeira TJPG, Miranda MJB, Moraes MO, Ferreira GE, Parente EA, Picollo NS. Characterization of the microbiota of the skin and oral cavity of Oreochromis niloticus. J Health Biol Sci. 2016 Abr-Jun; 4(3):193-197. J. Health Biol Sci. 2016; 4(3):193-197

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J. Health Biol Sci. 2016; 4(3):193-197 doi:10.12662/2317-3076jhbs.v4i3.767.p193-197.2016 ORIGINAL ARTICLE Characterization of the microbiota of the...

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